Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance.
Identifieur interne : 000189 ( Main/Exploration ); précédent : 000188; suivant : 000190Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance.
Auteurs : Jun Yao [République populaire de Chine] ; Zedan Shen [République populaire de Chine] ; Yanli Zhang [République populaire de Chine] ; Xia Wu [République populaire de Chine] ; Jianhui Wang [République populaire de Chine] ; Gang Sa [République populaire de Chine] ; Yuhong Zhang [République populaire de Chine] ; Huilong Zhang [République populaire de Chine] ; Chen Deng [République populaire de Chine] ; Jian Liu [République populaire de Chine] ; Siyuan Hou [République populaire de Chine] ; Ying Zhang [République populaire de Chine] ; Yinan Zhang [République populaire de Chine] ; Nan Zhao [République populaire de Chine] ; Shurong Deng [République populaire de Chine] ; Shanzhi Lin [République populaire de Chine] ; Rui Zhao [République populaire de Chine] ; Shaoliang Chen [République populaire de Chine]Source :
- Journal of experimental botany [ 1460-2431 ] ; 2020.
Abstract
Plasma membrane proton pumps play a crucial role in maintaining ionic homeostasis in salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica roots and leaves. We isolated a 2022 bp promoter fragment upstream of the translational start of PeHA1 from P. euphratica. The promoter-reporter construct PeHA1-pro::GUS was transferred to tobacco plants, demonstrating that β-glucuronidase activities increased in root, leaf, and stem tissues under salt stress. DNA affinity purification sequencing revealed that PeWRKY1 protein targeted the PeHA1 gene. We assessed the salt-induced transcriptional response of PeWRKY1 and its interaction with PeHA1 in P. euphratica. PeWRKY1 binding to the PeHA1 W-box in the promoter region was verified by a yeast one-hybrid assay, EMSA, luciferase reporter assay, and virus-induced gene silencing. Transgenic tobacco plants overexpressing PeWRKY1 had improved expression of NtHA4, which has a cis-acting W-box in the regulatory region, and improved H+ pumping activity in both in vivo and in vitro assays. We conclude that salt stress up-regulated PeHA1 transcription due to the binding of PeWRKY1 to the W-box in the promoter region of PeHA1. Thus, we conclude that enhanced H+ pumping activity enabled salt-stressed plants to retain Na+ homeostasis.
DOI: 10.1093/jxb/erz493
PubMed: 31680166
PubMed Central: PMC7031066
Affiliations:
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type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhao, Nan" sort="Zhao, Nan" uniqKey="Zhao N" first="Nan" last="Zhao">Nan Zhao</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Deng, Shurong" sort="Deng, Shurong" uniqKey="Deng S" first="Shurong" last="Deng">Shurong Deng</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, The Research Institute of Forestry, Chinese Academy of Forestry, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, The Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Lin, Shanzhi" sort="Lin, Shanzhi" uniqKey="Lin S" first="Shanzhi" last="Lin">Shanzhi Lin</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhao, Rui" sort="Zhao, Rui" uniqKey="Zhao R" first="Rui" last="Zhao">Rui Zhao</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chen, Shaoliang" sort="Chen, Shaoliang" uniqKey="Chen S" first="Shaoliang" last="Chen">Shaoliang Chen</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by 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tolerance.</title><author><name sortKey="Yao, Jun" sort="Yao, Jun" uniqKey="Yao J" first="Jun" last="Yao">Jun Yao</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Shen, Zedan" sort="Shen, Zedan" uniqKey="Shen Z" first="Zedan" last="Shen">Zedan Shen</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>South China Botanical Garden, Chinese Academy of Sciences, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Zhang, Yanli" sort="Zhang, Yanli" uniqKey="Zhang Y" first="Yanli" last="Zhang">Yanli Zhang</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wu, Xia" sort="Wu, Xia" uniqKey="Wu X" first="Xia" last="Wu">Xia Wu</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Jianhui" sort="Wang, Jianhui" uniqKey="Wang J" first="Jianhui" last="Wang">Jianhui Wang</name><affiliation wicri:level="1"><nlm:affiliation>Bluescape Hebei Biotech Co., Ltd, Baoding, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Bluescape Hebei Biotech Co., Ltd, Baoding</wicri:regionArea><wicri:noRegion>Baoding</wicri:noRegion></affiliation></author><author><name sortKey="Sa, Gang" sort="Sa, Gang" uniqKey="Sa G" first="Gang" last="Sa">Gang Sa</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Gansu Provincial Key Laboratory of Aridland Crop Sciences, Gansu Agricultural University, Lanzhou, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Gansu Provincial Key Laboratory of Aridland Crop Sciences, Gansu Agricultural University, Lanzhou</wicri:regionArea><wicri:noRegion>Lanzhou</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Yuhong" sort="Zhang, Yuhong" uniqKey="Zhang Y" first="Yuhong" last="Zhang">Yuhong Zhang</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, The Research Institute of Forestry, Chinese Academy of Forestry, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, The Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Huilong" sort="Zhang, Huilong" uniqKey="Zhang H" first="Huilong" last="Zhang">Huilong Zhang</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Deng, Chen" sort="Deng, Chen" uniqKey="Deng C" first="Chen" last="Deng">Chen Deng</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Jian" sort="Liu, Jian" uniqKey="Liu J" first="Jian" last="Liu">Jian Liu</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Hou, Siyuan" sort="Hou, Siyuan" uniqKey="Hou S" first="Siyuan" last="Hou">Siyuan Hou</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Ying" sort="Zhang, Ying" uniqKey="Zhang Y" first="Ying" last="Zhang">Ying Zhang</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Yinan" sort="Zhang, Yinan" uniqKey="Zhang Y" first="Yinan" last="Zhang">Yinan Zhang</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Forestry Institute of New Technology, Chinese Academy of Forestry, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Forestry Institute of New Technology, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhao, Nan" sort="Zhao, Nan" uniqKey="Zhao N" first="Nan" last="Zhao">Nan Zhao</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Deng, Shurong" sort="Deng, Shurong" uniqKey="Deng S" first="Shurong" last="Deng">Shurong Deng</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, The Research Institute of Forestry, Chinese Academy of Forestry, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, The Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Lin, Shanzhi" sort="Lin, Shanzhi" uniqKey="Lin S" first="Shanzhi" last="Lin">Shanzhi Lin</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhao, Rui" sort="Zhao, Rui" uniqKey="Zhao R" first="Rui" last="Zhao">Rui Zhao</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chen, Shaoliang" sort="Chen, Shaoliang" uniqKey="Chen S" first="Shaoliang" last="Chen">Shaoliang Chen</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Journal of experimental botany</title><idno type="eISSN">1460-2431</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plasma membrane proton pumps play a crucial role in maintaining ionic homeostasis in salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica roots and leaves. We isolated a 2022 bp promoter fragment upstream of the translational start of PeHA1 from P. euphratica. The promoter-reporter construct PeHA1-pro::GUS was transferred to tobacco plants, demonstrating that β-glucuronidase activities increased in root, leaf, and stem tissues under salt stress. DNA affinity purification sequencing revealed that PeWRKY1 protein targeted the PeHA1 gene. We assessed the salt-induced transcriptional response of PeWRKY1 and its interaction with PeHA1 in P. euphratica. PeWRKY1 binding to the PeHA1 W-box in the promoter region was verified by a yeast one-hybrid assay, EMSA, luciferase reporter assay, and virus-induced gene silencing. Transgenic tobacco plants overexpressing PeWRKY1 had improved expression of NtHA4, which has a cis-acting W-box in the regulatory region, and improved H+ pumping activity in both in vivo and in vitro assays. We conclude that salt stress up-regulated PeHA1 transcription due to the binding of PeWRKY1 to the W-box in the promoter region of PeHA1. Thus, we conclude that enhanced H+ pumping activity enabled salt-stressed plants to retain Na+ homeostasis.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">31680166</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN><JournalIssue CitedMedium="Internet"><Volume>71</Volume><Issue>4</Issue><PubDate><Year>2020</Year><Month>02</Month><Day>19</Day></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J Exp Bot</ISOAbbreviation></Journal><ArticleTitle>Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance.</ArticleTitle><Pagination><MedlinePgn>1527-1539</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/erz493</ELocationID><Abstract><AbstractText>Plasma membrane proton pumps play a crucial role in maintaining ionic homeostasis in salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica roots and leaves. We isolated a 2022 bp promoter fragment upstream of the translational start of PeHA1 from P. euphratica. The promoter-reporter construct PeHA1-pro::GUS was transferred to tobacco plants, demonstrating that β-glucuronidase activities increased in root, leaf, and stem tissues under salt stress. DNA affinity purification sequencing revealed that PeWRKY1 protein targeted the PeHA1 gene. We assessed the salt-induced transcriptional response of PeWRKY1 and its interaction with PeHA1 in P. euphratica. PeWRKY1 binding to the PeHA1 W-box in the promoter region was verified by a yeast one-hybrid assay, EMSA, luciferase reporter assay, and virus-induced gene silencing. Transgenic tobacco plants overexpressing PeWRKY1 had improved expression of NtHA4, which has a cis-acting W-box in the regulatory region, and improved H+ pumping activity in both in vivo and in vitro assays. We conclude that salt stress up-regulated PeHA1 transcription due to the binding of PeWRKY1 to the W-box in the promoter region of PeHA1. Thus, we conclude that enhanced H+ pumping activity enabled salt-stressed plants to retain Na+ homeostasis.</AbstractText><CopyrightInformation>© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yao</LastName><ForeName>Jun</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Zedan</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Yanli</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Xia</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jianhui</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Bluescape Hebei Biotech Co., Ltd, Baoding, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sa</LastName><ForeName>Gang</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Gansu Provincial Key Laboratory of Aridland Crop Sciences, Gansu Agricultural University, Lanzhou, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Yuhong</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, The Research Institute of Forestry, Chinese Academy of Forestry, Beijing, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Huilong</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Deng</LastName><ForeName>Chen</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Jian</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hou</LastName><ForeName>Siyuan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Ying</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Yinan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Forestry Institute of New Technology, Chinese Academy of Forestry, Beijing, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Nan</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Deng</LastName><ForeName>Shurong</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, The Research Institute of Forestry, Chinese Academy of Forestry, Beijing, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Shanzhi</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Rui</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Shaoliang</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology (Box 162), Beijing Forestry University, Beijing, PR China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>Dryad</DataBankName><AccessionNumberList><AccessionNumber>10.5061/dryad.83ng085</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">DNA affinity purification sequencing</Keyword><Keyword MajorTopicYN="Y">H+-ATPase</Keyword><Keyword MajorTopicYN="Y">NaCl</Keyword><Keyword MajorTopicYN="Y">PeWRKY1</Keyword><Keyword MajorTopicYN="Y">W-box</Keyword><Keyword MajorTopicYN="Y">electrophoretic mobility shift assay</Keyword><Keyword MajorTopicYN="Y">luciferase reporter assay</Keyword><Keyword MajorTopicYN="Y">non-invasive microtest technique</Keyword><Keyword MajorTopicYN="Y">virus-induced gene silencing</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>07</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>11</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>11</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>11</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Yao, Jun" sort="Yao, Jun" uniqKey="Yao J" first="Jun" last="Yao">Jun Yao</name></noRegion><name sortKey="Chen, Shaoliang" sort="Chen, Shaoliang" uniqKey="Chen S" first="Shaoliang" last="Chen">Shaoliang Chen</name><name sortKey="Deng, Chen" sort="Deng, Chen" uniqKey="Deng C" first="Chen" last="Deng">Chen Deng</name><name sortKey="Deng, Shurong" sort="Deng, Shurong" uniqKey="Deng S" first="Shurong" last="Deng">Shurong Deng</name><name sortKey="Deng, Shurong" sort="Deng, Shurong" uniqKey="Deng S" first="Shurong" last="Deng">Shurong Deng</name><name sortKey="Hou, Siyuan" sort="Hou, Siyuan" uniqKey="Hou S" first="Siyuan" last="Hou">Siyuan Hou</name><name sortKey="Lin, Shanzhi" sort="Lin, Shanzhi" uniqKey="Lin S" first="Shanzhi" last="Lin">Shanzhi Lin</name><name sortKey="Liu, Jian" sort="Liu, Jian" uniqKey="Liu J" first="Jian" last="Liu">Jian Liu</name><name sortKey="Sa, Gang" sort="Sa, Gang" uniqKey="Sa G" first="Gang" last="Sa">Gang Sa</name><name sortKey="Sa, Gang" sort="Sa, Gang" uniqKey="Sa G" first="Gang" last="Sa">Gang Sa</name><name sortKey="Shen, Zedan" sort="Shen, Zedan" uniqKey="Shen Z" first="Zedan" last="Shen">Zedan Shen</name><name sortKey="Shen, Zedan" sort="Shen, Zedan" uniqKey="Shen Z" first="Zedan" last="Shen">Zedan Shen</name><name sortKey="Wang, Jianhui" sort="Wang, Jianhui" uniqKey="Wang J" first="Jianhui" last="Wang">Jianhui Wang</name><name sortKey="Wu, Xia" sort="Wu, Xia" uniqKey="Wu X" first="Xia" last="Wu">Xia Wu</name><name sortKey="Zhang, Huilong" sort="Zhang, Huilong" uniqKey="Zhang H" first="Huilong" last="Zhang">Huilong Zhang</name><name sortKey="Zhang, Yanli" sort="Zhang, Yanli" uniqKey="Zhang Y" first="Yanli" last="Zhang">Yanli Zhang</name><name sortKey="Zhang, Yinan" sort="Zhang, Yinan" uniqKey="Zhang Y" first="Yinan" last="Zhang">Yinan Zhang</name><name sortKey="Zhang, Yinan" sort="Zhang, Yinan" uniqKey="Zhang Y" first="Yinan" last="Zhang">Yinan Zhang</name><name sortKey="Zhang, Ying" sort="Zhang, Ying" uniqKey="Zhang Y" first="Ying" last="Zhang">Ying Zhang</name><name sortKey="Zhang, Yuhong" sort="Zhang, Yuhong" uniqKey="Zhang Y" first="Yuhong" last="Zhang">Yuhong Zhang</name><name sortKey="Zhang, Yuhong" sort="Zhang, Yuhong" uniqKey="Zhang Y" first="Yuhong" last="Zhang">Yuhong Zhang</name><name sortKey="Zhao, Nan" sort="Zhao, Nan" uniqKey="Zhao N" first="Nan" last="Zhao">Nan Zhao</name><name sortKey="Zhao, Rui" sort="Zhao, Rui" uniqKey="Zhao R" first="Rui" last="Zhao">Rui Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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